Alanine Scanning Mutagenesis Pipeline

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alanine_scanning_pipeline is a Claude Code skill that implements a complete alanine scanning mutagenesis pipeline: it designs alanine-substituted mutants from an input sequence, computes their biochemical parameters, predicts drug-target interactions, and compares the resulting protein properties. The workflow runs across 4 tools from 3 servers — AlanineScanningDesigner, ComputeProtPara, PredictDrugTargetInteraction, and calculate_protein_sequence_properties. Researchers in protein biochemistry and protein engineering can use it to systematically identify residues critical for function or binding and guide protein optimization. The skill accepts an amino acid sequence and produces a structured comparison of physicochemical properties and interaction profiles across all generated mutants.